Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Phylogenetic Properties of 50 Nuclear Loci in Medicago (Leguminosae) Generated Using Multiplexed Sequence Capture and Next-Generation Sequencing
Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden.
Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden.
Swedish Museum of Natural History, Department of Botany.ORCID iD: 0000-0001-5199-7128
Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden.
Show others and affiliations
2014 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 9, no 10Article in journal (Refereed) Published
Abstract [en]

Next-generation sequencing technology has increased the capacity to generate molecular data for plant biological research,including phylogenetics, and can potentially contribute to resolving complex phylogenetic problems. The evolutionaryhistory of Medicago L. (Leguminosae: Trifoliae) remains unresolved due to incongruence between published phylogenies.Identification of the processes causing this genealogical incongruence is essential for the inference of a correct speciesphylogeny of the genus and requires that more molecular data, preferably from low-copy nuclear genes, are obtainedacross different species. Here we report the development of 50 novel LCN markers in Medicago and assess the phylogeneticproperties of each marker. We used the genomic resources available for Medicago truncatula Gaertn., hybridisation-basedgene enrichment (sequence capture) techniques and Next-Generation Sequencing to generate sequences. This alternativeproves to be a cost-effective approach to amplicon sequencing in phylogenetic studies at the genus or tribe level andallows for an increase in number and size of targeted loci. Substitution rate estimates for each of the 50 loci are provided,and an overview of the variation in substitution rates among a large number of low-copy nuclear genes in plants ispresented for the first time. Aligned sequences of major species lineages of Medicago and its sister genus are made availableand can be used in further probe development for sequence-capture of the same markers.

Place, publisher, year, edition, pages
2014. Vol. 9, no 10
National Category
Biological Systematics
Research subject
Diversity of life
Identifiers
URN: urn:nbn:se:nrm:diva-899DOI: 10.1371/journal.pone.0109704OAI: oai:DiVA.org:nrm-899DiVA, id: diva2:766941
Funder
Swedish Research Council, 2009-5206Available from: 2014-11-28 Created: 2014-11-28 Last updated: 2023-10-16Bibliographically approved

Open Access in DiVA

fulltext(456 kB)228 downloads
File information
File name FULLTEXT01.pdfFile size 456 kBChecksum SHA-512
de03aab6c4ffd49612d6e43ef07a5b20b1aeed09e8c0a63226f1d4069801a2f900708fd3f20b14e4bfe3665de576e3ae0838b882d03639fa404be31d776eb941
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Nylinder, Nylinder
By organisation
Department of Botany
In the same journal
PLOS ONE
Biological Systematics

Search outside of DiVA

GoogleGoogle Scholar
Total: 228 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 201 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf