In this study we identify Agrotis exclamationis (Linnaeus, 1758) as a host species for the bee y Villa hottentotta (Linnaeus, 1758) in Sweden. Host use and speci city for bee y species are generally very poorly known, why the hatching of a bee y of the genus Villa from an unknown Noctuid pupa caught our attention. The parasitized Noctuid pupa was found in a garden in Staffanstorp, Skåne (Sweden), in May 2015 and kept in a jar to hatch. The bee y hatched in June leaving two empty exuviae in the jar. DNA was extracted sepa- rately from both excuviae to identify the y and the host using DNA Barcoding. A 600+ bp long sequence of the gene Cytochrome oxidase subunit 1 was sequenced for both samples and queried against the reference library BOLD (www.boldsystems.org). The Noctuid host pupa was unambiguously identi ed as the common Noctuid species Agrotis exclamationis. The sequence was identical to the most common haplotype over much of Europe. The bee y pupa was identi ed as Villa hottentotta, the most common Villa species in Sweden. This added a new Noctuid species to the list of known hosts for V. hottentotta which also includes several other Noctuid genera as well as a Geometrid moth. Belonging to the sand chamber group of bee ies where the female scatter the eggs on the ground while hovering, the active host-seeking rst instar planidium larvae bene ts from having a wide host range to potentially encounter in the substrate zone.