Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Metagenomic Analysis from the Interior of a Speleothem in Tjuv-Ante's Cave, Northern Sweden
Natural History Museum of Denmark.ORCID iD: 0000-0002-1126-8219
Swedish Museum of Natural History, Department of Botany.ORCID iD: 0000-0003-4316-9183
Swedish Museum of Natural History, Department of Paleobiology. Nordic Center for Earth Evolution (NordCEE).
University of Copenhagen.ORCID iD: 0000-0003-1285-4671
Show others and affiliations
2016 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, no 3, 1-23 p., e015177Article in journal (Refereed) Published
Abstract [en]

Speleothems are secondary mineral deposits normally formed by water supersaturated with calcium carbonate percolating into underground caves, and are often associated with low-nutrient and mostly non-phototrophic conditions. Tjuv-Ante’s cave is a shallow-depth cave formed by the action of waves, with granite and dolerite as major components, and opal-A and calcite as part of the speleothems, making it a rare kind of cave. We generated two DNA shotgun sequencing metagenomic datasets from the interior of a speleothem from Tjuv-Ante’s cave representing areas of old and relatively recent speleothem formation. We used these datasets to perform i) an evaluation of the use of these speleothems as past biodiversity archives, ii) functional and taxonomic profiling of the speleothem’s different formation periods, and iii) taxonomic comparison of the metagenomic results to previous microscopic analyses from a nearby speleothem of the same cave. Our analyses confirm the abundance of Actinobacteria and fungi as previously reported by microscopic analyses on this cave, however we also discovered a larger biodiversity. Interestingly, we identified photosynthetic genes, as well as genes related to iron and sulphur metabolism, suggesting the presence of chemoautotrophs. Furthermore, we identified taxa and functions related to biomineralization. However, we could not confidently establish the use of this type of speleothems as biological paleoarchives due to the potential leaching from the outside of the cave and the DNA damage that we propose has been caused by the fungal chemical etching.

Place, publisher, year, edition, pages
2016. Vol. 11, no 3, 1-23 p., e015177
Keyword [en]
speleothem, cave, geomicrobiology, Sweden, granite, dolerite, fungi, Actinobacteria, metagenomics
National Category
Geosciences, Multidisciplinary
Research subject
Diversity of life
Identifiers
URN: urn:nbn:se:nrm:diva-2258DOI: 10.1371/journal.pone.0151577OAI: oai:DiVA.org:nrm-2258DiVA: diva2:1070242
Available from: 2017-01-31 Created: 2017-01-31 Last updated: 2017-01-31Bibliographically approved

Open Access in DiVA

fulltext(2098 kB)9 downloads
File information
File name FULLTEXT01.pdfFile size 2098 kBChecksum SHA-512
a1f176e13dc01a9a33bdb8cc090c16a58904d4f4cece50edb0e4f8120d392d9480919774f8d6c0f9ec64b8b37a193540b62d6c15fb5aa919ed1c3c00e9fed286
Type fulltextMimetype application/pdf

Other links

Publisher's full texthttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0151577

Search in DiVA

By author/editor
Zepeda Mendoza, Marie LisandraLundberg, JohannesIvarsson, MagnusCampos, PaulaNylander, Johan A. A.Sallstedt, ThereeseDalén, Love
By organisation
Department of BotanyDepartment of PaleobiologyDepartment of Bioinformatics and Genetics
In the same journal
PLoS ONE
Geosciences, Multidisciplinary

Search outside of DiVA

GoogleGoogle Scholar
Total: 9 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 11 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf