A spectacular feature of the Arabian-Nubian Shield (ANS) is the abundance of well-exposed and extensive Neoproterozoic dike swarms of variable compositions. Most of these dikes are late to post-orogenic with respect to the East African Orogen (EAO) and are unmetamorphosed. We dated a composite dike with latite margins and a rhyolite core (607 ± 6 Ma, U-Pb), a biotite rhyolite dike (600 ± 4 Ma, 40Ar/39Ar age of biotite), an andesite dike (594 ± 3, 40Ar/39Ar age of amphibole) and a dolerite dike (~579 Ma, 40Ar/39Ar whole rock total gas age). We propose that the first three dikes represent one generation that was emplaced at different episodes extending between 607 and 590 Ma. Time and composition equivalent dikes are common in the northern ANS. The dikes crosscut late collisional granitoids and geochemically display a subduction-related character as evidenced by a negative Nb-Ta anomaly. These dikes are absent in the alkali feldspar A-type Humrat Syenogranite dated at 586 ± 5 Ma in Jordan and equivalent rocks in the northern ANS, which are crosscut only by the (~579 Ma) dolerite dikes. The within-plate character of the dolerite dikes is supported by the absence of the Nb-Ta anomaly and high field strength element geochemistry. We propose that the dolerite dikes are a generation, distinct from the ~607–590 Ma dikes, that reflects a change in tectonic regime and represents the last magmatic activity of the Neoproterozoic in the northern ANS. The ages of the dikes dated in this study agree with the published age range of the transitional stage from late orogenic calc-alkaline to extensional alkaline tectono-magmatic setting for the ANS. We propose that the magmatic activity was terminated ~50 m.y. before the age of the Cambrian unconformity at ~530 Ma. Correlation with ages of dikes and magmatic rocks in the northern ANS favors this supposition. The dike geochemistry and geochronology are compatible with a tectonic model that involves mantle lithosphere delamination from below the northern ANS after a significant crust-mantle thickening caused by the EAO, followed by thermal relaxation, subsidence and gradual denudation until the age of the unconformity at ~530 Ma.