In the summer of 2016, the International Ocean Discovery Program (IODP) Expedition 364 coredthrough the post-impact strata of the end-Cretaceous Chicxulub impact crater, Mexico. Core sampleswere collected from the post-impact successions for terrestrial palynological analysis, yielding a rareDanian to Ypresian high-resolution palynological assemblage. This record constitutes one of the firstPalaeocene and Ypresian palynological assemblages from Central America or Mexico, representing amore coastal lowland palaeoenvironment than previous studies from mainland Mexico. Although theabundance of pollen and spores is very low in the Palaeocene carbonates, abundance increases in themore organic-rich shale layers representing the Palaeocene–Eocene Thermal Maximum (PETM) andlater Ypresian. The spores and gymnosperm pollen identified from IODP 364, although rare comparedto the angiosperm pollen, are a diverse mix of cosmopolitan taxa, as well as some characteristic of fossilCentral American assemblages (e.g. Selaginellaceae), and others previously identified from thePaleogene northern Gulf of Mexico coastal plain. The assemblage generally indicates the presence ofnearby moist to seasonally dry lowland tropical forest, with some taxa suggestive of higher elevationforests. Ephedroid pollen grains may be indicative of the presence of more arid conditions.