We report the development of 14 novel polymorphic microsatellite markers cloned from theWhite-tailed Sea Eagle, Haliaeetus albicilla, a formerly threatened raptor that has receivedmuch conservation attention throughout Eurasia. We also present a protocol for multiplexpolymerase chain reaction (PCR) amplification of the loci. Among 40 unrelated H. albicillaindividuals from southern Sweden, the markers produced two to eight alleles per locus,and average observed and expected heterozygosities were 0.463 and 0.468, respectively. Wefurther present five microsatellite markers that appeared monomorphic in H. albicilla, butwhich may be of interest for use in other raptor species