Fungi in subsurface and underground environments is a rather new and underexplored field within mycology, and our understanding of these communities are sparse. However, available reports indicate a high fungal diversity with a large poriton of undescribed taxa in these settings. Here we investigate the fungal presence in the Falun copper mine, Sweden, with the aim to identify fungi useable in bioremediation processes. Thirteen of fourteen samples were successfully cultured, and nine isolates obtained in total. Further culturing and investigations are needed to understand the bioremediation abilities among the fungi, but this first reort indicates a high abundance and diversity of fungi in the underground Falun copper mine.