Here, we describe and illustrate by transmitted light and scanning electron microscopy the first Australian Jurassic megaspore assemblages. The megaspores and other mesofossils were isolated from terrestrial deposits of the Marburg Subgroup (late Pliensbachian) at Inverleigh Quarry, Clarence-Moreton Basin, eastern Australia. Nine megaspore taxa are identified including one new species: Paxillitriletes rainei. Miospore assemblages recovered from the same samples at Inverleigh reveal a slightly higher diversity of lycophyte microspores. The collective megaspore suite from Inverleigh shares several genera with mid-Mesozoic assemblages from widely distributed parts of the world, but most of the Inverleigh species have subtle morphological differences from congeneric forms elsewhere. The megaspores accumulated in fluvial floodplain facies and are associated with mostly dissociated isoetalean leaf debris. Other mesofossils in the sampled interval include annelid egg cases, dispersed seeds and charcoal. Invertebrate burrows and possible vertebrate tracks also occur in this succession. Lycophyte macrofossils are otherwise known from only two other Australian Jurassic deposits. The richness of the megaspore and microspore suites attest to a significant diversity of lycophytes in the Australian Jurassic floras not hitherto appreciated from described macrofloras