Sixty-seven species of the subfamily Pyraustinae from 17 genera and two tribes are listed. Anania verbascalis verbascalis ([Denis & Schiffermüller], 1949) is considered as a senior synonym of A. verbascalis parvalis (Osthelder, 1935) syn. n.; Pyrausta virginalis Duponchel, 1832 as a senior synonym of P. perkeo Amsel, 1970 syn. n.; and P. ferrealis (Hampson, 1900) as a senior synonym of P. euergestalis Amsel, 1954 syn. n. Thirteen species, namely Anania coronata (Hufnagel, 1767), A. murcialis (Ragonot, 1895), A. terrealis (Treitschke, 1829), Pyrausta ferrealis (Hampson, 1900), P. armeniaca Slamka, 2013, P. zeitunalis Caradja, 1916, P. cingulata (Linnaeus, 1758), P. delicatalis Caradja, 1916, P. tatarica Kemal et al., 2020, Loxostege sedakowialis (Eversmann, 1852), L. wagneri Zerny in Wagner, 1929, L. mucosalis (Herrich-Schäffer, 1848) and L. peltaloides (Rebel in Wagner, 1932), are newly reported from Iran. Additionally, a redescription of the male of P. delicatalis and the female of L. sedakowialis, as well as description of the hitherto unknown female of P. delicatalis are presented. The intraspecific variations of the species if present are discussed and additional new diagnostic characters to separate the closely related species are presented. Data on the geographical distribution of the Iranian species of this subfamily are provided.